Optimization of staining with SYTO 9/propidium iodide: interplay, kinetics and impact on Brevibacillus brevis
Fluorophores SYTO 9 and propidium iodide (PI) are extensively applied in medicine, food industry and environmental monitoring to trays assess the viability of bacteria.However, the actual performance of these dyes remains largely unknown.In addition, their effects on the physiology of cells have not been elucidated.Here we characterized the effects